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(a) Anti-gE IgG1 and (b) IgG2c titers at week 4. (b) Ratio of anti-gE IgG2c to IgG1 at week 4. Ratio below 1 indicates more Th2 skewed response. (d) Timepoint of serum collection for circulating cytokine measurement. All serum samples were collected 24 hours after the injection. (e) <t>IL6</t> concentration of young and aged mice 24 hours after injection at week 0 and 8. One-way ANOVA was used for multiple comparisons, and only p-values below 0.05 are displayed.
Mouse Il6 Serum Cytokine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(a) Anti-gE IgG1 and (b) IgG2c titers at week 4. (b) Ratio of anti-gE IgG2c to IgG1 at week 4. Ratio below 1 indicates more Th2 skewed response. (d) Timepoint of serum collection for circulating cytokine measurement. All serum samples were collected 24 hours after the injection. (e) <t>IL6</t> concentration of young and aged mice 24 hours after injection at week 0 and 8. One-way ANOVA was used for multiple comparisons, and only p-values below 0.05 are displayed.
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(a) Anti-gE IgG1 and (b) IgG2c titers at week 4. (b) Ratio of anti-gE IgG2c to IgG1 at week 4. Ratio below 1 indicates more Th2 skewed response. (d) Timepoint of serum collection for circulating cytokine measurement. All serum samples were collected 24 hours after the injection. (e) <t>IL6</t> concentration of young and aged mice 24 hours after injection at week 0 and 8. One-way ANOVA was used for multiple comparisons, and only p-values below 0.05 are displayed.
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inflammatory cytokines interleukin 6  (Cusabio)
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(a) Anti-gE IgG1 and (b) IgG2c titers at week 4. (b) Ratio of anti-gE IgG2c to IgG1 at week 4. Ratio below 1 indicates more Th2 skewed response. (d) Timepoint of serum collection for circulating cytokine measurement. All serum samples were collected 24 hours after the injection. (e) <t>IL6</t> concentration of young and aged mice 24 hours after injection at week 0 and 8. One-way ANOVA was used for multiple comparisons, and only p-values below 0.05 are displayed.
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(a) Anti-gE IgG1 and (b) IgG2c titers at week 4. (b) Ratio of anti-gE IgG2c to IgG1 at week 4. Ratio below 1 indicates more Th2 skewed response. (d) Timepoint of serum collection for circulating cytokine measurement. All serum samples were collected 24 hours after the injection. (e) <t>IL6</t> concentration of young and aged mice 24 hours after injection at week 0 and 8. One-way ANOVA was used for multiple comparisons, and only p-values below 0.05 are displayed.
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il6 cytokine  (PeproTech)
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(a) Anti-gE IgG1 and (b) IgG2c titers at week 4. (b) Ratio of anti-gE IgG2c to IgG1 at week 4. Ratio below 1 indicates more Th2 skewed response. (d) Timepoint of serum collection for circulating cytokine measurement. All serum samples were collected 24 hours after the injection. (e) <t>IL6</t> concentration of young and aged mice 24 hours after injection at week 0 and 8. One-way ANOVA was used for multiple comparisons, and only p-values below 0.05 are displayed.
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(a) Anti-gE IgG1 and (b) IgG2c titers at week 4. (b) Ratio of anti-gE IgG2c to IgG1 at week 4. Ratio below 1 indicates more Th2 skewed response. (d) Timepoint of serum collection for circulating cytokine measurement. All serum samples were collected 24 hours after the injection. (e) IL6 concentration of young and aged mice 24 hours after injection at week 0 and 8. One-way ANOVA was used for multiple comparisons, and only p-values below 0.05 are displayed.

Journal: bioRxiv

Article Title: Sustained Delivery of a Shingles Subunit Vaccine Overcomes Age-Related Declines in Humoral and Cellular Immunity Relative to Shingrix

doi: 10.64898/2026.03.11.711122

Figure Lengend Snippet: (a) Anti-gE IgG1 and (b) IgG2c titers at week 4. (b) Ratio of anti-gE IgG2c to IgG1 at week 4. Ratio below 1 indicates more Th2 skewed response. (d) Timepoint of serum collection for circulating cytokine measurement. All serum samples were collected 24 hours after the injection. (e) IL6 concentration of young and aged mice 24 hours after injection at week 0 and 8. One-way ANOVA was used for multiple comparisons, and only p-values below 0.05 are displayed.

Article Snippet: For cytokine ELISA, mouse IL6 serum cytokine ELISA kit was purchased from R&D systems and used following the manufacturer’s guide.

Techniques: Injection, Concentration Assay